Is green metallic sheen in emb agar accurate to assist the Escherichia coli identification?

Autores/as

DOI:

https://doi.org/10.21727/rpu.v15i4.4520

Resumen

Escherichia coli is an essential bacteria as a human and animal pathogen, although this species can also be disseminated in the environment. The clinical laboratories use EMB agar (Eosin Methylene Blue) to identify E. coli from the observation of metallic green sheen. However, other bacterial species may show sheen when sown on EMB agar, or in some cases, the pH sample influences the presence of this sheen by E. coli. In this study, MALDI-TOF was used as the gold standard to detect E. coli from human, animal, and environmental samples by analyzing the presence of green glow on EMB agar. The positive predictive values (PPV) ​​were higher (above 85%) in human, animal, and environmental samples studied, and the negative predictive value (NPV) was variable between the samples. Specificity and sensitivity were also variables suggesting the influence of the pH of the samples in the presence of metallic green brightness. This work demonstrated that it is necessary to implement biochemical tests or, when possible, molecular assays to confirm E. coli since only the observation of the production of metallic green glow in EMB can generate false negative results in the identification of E. coli. 

Keywords: Biochemical tests; Escherichia coli; Laboratory Analysis; MALDI-TOF.

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Biografía del autor/a

Hosana Dau Ferreira de Souza, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Discente de Doutorado pelo Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brasil. 

Paula Fernanda Alves Ferreira, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Discente de Doutorado pelo Programa de Pós-Graduação em Agronomia - Ciências do Solo, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro.

Thereza Cristina da Costa Patricio, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Discente de Mestrado pelo Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brasil

Gabrielli Stefaninni Santiago, Faculdade Anhanguera

Docente da Faculdade Anhanguera, São José dos Campos, São Paulo, Brasil.

Irene da Silva Coelho, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Docente Associada, Departamento de Microbiologia e Imunologia Veterinária - Universidade federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brasil.

Miliane Moreira Soares de Souza, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Docente Titular, Departamento de Microbiologia e Imunologia Veterinária - Universidade federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brasil.

Kayo Cesar Bianco Fernandes, National Institute of Quality Control in Health - INCQS

National Institute of Quality Control in Health - INCQS, Oswaldo Cruz Foundation - FIOCRUZ, Rio de Janeiro, RJ 4365, Brazil.

Shana de Mattos de Oliveira Coelho, Universidade Federal Rural do Rio de Janeiro (UFRRJ)

Docente Associada, Departamento de Microbiologia e Imunologia Veterinária - Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brasil.

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Publicado

2025-01-13

Número

Vol. 15 Núm. 4 (2024): Revista Pró-UniverSUS V 15 N 4

Sección

Articles

Licencia

Derechos de autor 2024 Hosana Dau Ferreira de Souza, Paula Fernanda Alves Ferreira, Thereza Cristina da Costa Patricio, Gabrielli Stefaninni Santiago, Irene da Silva Coelho, Miliane Moreira Soares de Souza, Kayo Cesar Bianco Fernandes, Shana de Mattos de Oliveira Coelho

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